Objectives: The purpose of this study was to examine the relationship between uric acid level and chronic kidney disease (CKD) in the middle-aged and elderly population in Southern Taiwan. Methods: This was a cross-sectional study of 9,450 people recruited from a population that received free adult health examinations provided by a hospital in a district in Kaohsiung City between July 2005 and July 2011. CKD diagnosis standards followed those set by the U.S. National Kidney Foundation kidney disease outcomes quality initiative (K/DOQI) in 2002. The relationship between hyperuricemia and CKD was examined and multiple logistic regression analysis was used to identify factors affecting CKD. Results: The prevalence of hyperuricemia amongst patients with CKD was 45.1%, with the level of uric acid(UA) concentration increasing at an abnormal rate at different stages of CKD. The results of multiple logistic regression analysis of UAconcentrations showed that men, aged 65 and above and metabolic syndrome were at risk of CKD. On the other hand, daily consumption of fruits and vegetables served as a protective factor. Based on the different categories of uric acid, we discovered that regardless of the regression model used, whether I, II, or III, the risk of contracting CKD increased as the UA concentration level increased.